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By def-inition, primary culture indicates that the biological materialcomes directly from the tissue and is grown on a culture sur-face (e.g., dish or flask) without additional passaging.passaging is a process by which cells that have grown toconfluence (i.e., grown until they form a monolayer thatcompletely fills up the available surface) are removed fromtheir growth surface, diluted with culture medium, and thenreapplied to a new culture surface to continue to grow.Because many cellular processes change as a consequenceof culture conditions, many investigators prefer to use cellsin primary culture as these will exhibit properties andfunctions closest to those of the in vivo tissue.

(2006a) Clinicopath-ological and imaging correlates of progressive aphasia andapraxia of speech.

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